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通过激光斑纹流动评估正常大鼠视神经头血流的纵向变化

Wada Y, Higashide T, Nagata A, et al.

期刊名称:Investigative ophthalmology & visual science

卷期:2016年第57卷第13期

摘要

目的:旨在研究大鼠视神经末梢(ONH)的平均模糊率(MBR)纵向变化(通过激光散斑血流成像(LSFG)测量),以及MBR的重现性。方法:大鼠全身麻醉,30分钟后测量眼压(IOP),血压,眼睛灌注压(OPP),心率和LSFG。使用LSFG-Micro测定ONH中的平均模糊率,并将其细分为总面积(MA),血管区域(MV)和组织区域(MT)的MBR。在10,11,13,1920周重复检测平均模糊率,之后是每5周一次,直至60周龄。评估期间重复性,期间可复制性和期内可复制性。结果:期间重复性,期间可复制性和期内可复制性的MBR变异系数分别为0.3-6.2%,1.3-5.2%和5.8-30.4%。19周龄前的总面积,MVMT平均模糊率增加相似,后稳定至60周。下象限总面积平均模糊率明显高于19-55周颞侧。这些变化超出了相应的重复系数范围。实验期间眼压,血压,OPP均无明显变化。结论:大鼠ONH的平均模糊率随时间而变化,从10-19周龄增加,稳定至60周。总面积的平均模糊率呈现出区域差异:下象限高于颞侧象限。激光散斑血流成像- Micro可为评估大鼠ONH血流纵向变化提供可靠的信息。

Purpose:To investigate longitudinal changes in mean blur rate (MBR) measured by laser speckle flowgraphy (LSFG) in the rat optic nerve head (ONH), and the reproducibility of MBR.Methods:Rats were dilated under general anesthesia. Intraocular pressure (IOP), blood pressure, ocular perfusion pressure (OPP), heart rate, and LSFG were measured 30 minutes later. Mean blur rate in the ONH was determined using LSFG-Micro and was subdivided into MBR of the total area (MA), vessel region (MV), and tissue region (MT). Mean blur rate measurements were repeated at 10, 11, 13, 19, and 20 weeks, then every 5 weeks until 60 weeks of age. Intrasession repeatability, intrasession reproducibility, and intersession reproducibility were evaluated.Results:Coefficient of variation of MBR was 0.3 to 6.2%, 1.3 to 5.2%, and 5.8 to 30.4% for intrasession repeatability, intrasession reproducibility, and intersession reproducibility, respectively. Mean blur rate of the total area, MV, and MT increased similarly until 19 weeks of age, but stabilized thereafter until 60 weeks. Mean blur rate of the total area in the inferior quadrant was significantly higher than in the temporal quadrant from 19 to 55 weeks. These changes exceeded a range of corresponding coefficient of reproducibility. There were no significant changes in IOP, blood pressure, or OPP during the experimental period.Conclusions:Mean blur rate in the rat ONH changed over time, increased from 10 to 19 weeks of age, then stabilized until 60 weeks. Mean blur rate of the total area exhibited regional differences: higher in the inferior quadrant than in the temporal quadrant. Laser speckle flowgraphy-Micro may provide reliable information for evaluating longitudinal changes of rat ONH blood flow.


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