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短期光适应对人后照明瞳孔反应的影响

Joyce DS, Feigl B, Zele AJ

期刊名称:Investigative ophthalmology & visual science

卷期:2016年第57卷第13期

关键词:
瞳孔 短期 照明

摘要

目的:我们确定了短期光适应对瞳孔光反射和黑色素介导的后照明瞳孔反应(PIPR)的影响。我们评估了内外视网膜光感受器对黑暗适应瞳孔反应的影响。方法:在实验A中,黑色素激动刺激脉冲前(5-60秒)后(30秒)使用黑色素信号亚阈值到超阈值辐照度短波长光研究光适应。我们量化了暗(PIPR)和光(刺激瞳孔反应,PSPR)适应期间的瞳孔收缩和后刺激反应幅度。在实验B中,彩色的预刺激适应光对黑色素或视杆激发而言是单变量的。结果:当恢复至暗适应基线时,增加拟光持续时间和适应光的辐照度可增加瞳孔收缩幅度,但当恢复至光适应基线时,则可降低其幅度。黑色素激活上限阈值光适应增加了PIPR幅度,在更长的适应持续时间内有较大的变化,而随着辐照度的增加,PSPR幅度变得更加衰减,与持续时间无关。 Rod与黑视蛋白单变量适应并没有改变收缩幅度,而是增加了视杆状态下的PIPR振幅。在标准化至基线直径时,毫米级的瞳孔收缩与PIPR幅度之间的相关性被消除。结论:这些发现有助于标准化光适应和实验室和临床环境下瞳孔指标的选择。光和暗适应对瞳孔度量有相反的影响,应标准化至基线以最小化收缩和PIPR幅度之间的显着相关性。

Purpose:We determine the effect of short-term light adaptation on the pupil light reflex and the melanopsin mediated post-illumination pupil response (PIPR). Inner and outer retinal photoreceptor contributions to the dark-adapted pupil response were estimated.Methods:In Experiment A, light adaptation was studied using short wavelength lights ranging from subthreshold to suprathreshold irradiances for melanopsin signaling that were presented before (5-60 seconds) and after (30 seconds) a melanopsin-exciting stimulus pulse. We quantified the pupil constriction and the poststimulus response amplitudes during dark (PIPR) and light (poststimulus pupil response, PSPR) adaptation. In Experiment B, colored prestimulus adapting lights were univariant for melanopsin or rod excitation.Results:Increasing the prestimulus duration and irradiance of adapting lights increased the pupil constriction amplitude when normalized to the dark-adapted baseline but reduced its amplitude when normalized to the light-adapted baseline. Light adaptation at irradiances suprathreshold for melanopsin activation increased the PIPR amplitude, with larger changes at longer adaptation durations, whereas the PSPR amplitude became more attenuated with increasing irradiances, independent of duration. Rod versus melanospin univariant adaptation did not alter the constriction amplitude but increased the PIPR amplitude in the rod condition. Correlations between millimeter pupil constriction and PIPR amplitudes were eliminated when normalized to the baseline diameter.Conclusions:The findings have implications for standardizing light adaptation paradigms and the choice of pupil metrics in both laboratory and clinical settings. Light and dark adaptation have opposite effects on the pupil metrics, which should be normalized to baseline to minimize significant correlations between constriction and PIPR amplitudes.


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