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眼表疾病眼房水中细胞因子水平升高

Aketa, N., Yamaguchi, T., Asato, T., et al.

期刊名称:American journal of ophthalmology

卷期:2017年第184卷

摘要

摘要:目的:旨在评价眼表疾病患者房水中细胞因子和蛋白水平。设计:连续病例分析。方法:本研究共纳入14例(62.4±13.7岁)慢性眼表疾病(眼瘢痕性类天疱疮4例,化学性烧伤5例,热灼伤2例,Stevens-Johnson综合征2例,暴露性角膜炎1例), 无眼表疾病对照组14例(角膜瘢痕对照组)和30例接受白内障手术患者(健康对照)。在开始手术时收集AqH样品。通过多重珠免疫分析检测房水中的细胞因子水平(白细胞介素1α,IL-1β,IL-4IL-6IL-8IL-10IL-12p70IL-13IL-17A,单核细胞趋化蛋白[MCP] -1,干扰素IFN-α,IFN-γ,巨噬细胞炎性蛋白[MIP]-1α,MIP-1β,P-选择素,E-选择素,可溶性细胞间粘附分子[s-ICAM] -1,肿瘤坏死因子[TNF]-α,粒细胞 - 巨噬细胞集落刺激因子[GM-CSF]IFN-γ诱导蛋白[IP] -10)。结果:眼表疾病患者AqHIL-6IL-10IL-17AGM-CSFE-选择素,P-选择素和s-ICAM水平显着升高(pg / mL1696± IL-66.36±0.94P = .001; IL-66.34±0.94P = 0.001),分别为804,4.0±1.0,24.3±9.8,26.0±18.3,5150±1232,13122±72197914±2813 101.68±0.04P = 0.0006; IL-17A3.7±0.2P = .008; GM-CSF2.7±0.3P = .007; E-选择素:2093±37P = 0.0001; P-选择素:3658±137P = 0.0001; sICAM-11397±119P = 0.008)。 眼表疾病患者AqHIL-6IL-17AE-selectinP-selectin水平明显较角膜瘢痕患者增高(IL-644.1±15.0P = 0.0077; IL-17A4.1±0.7P = 0.034; E-选择素:2439±302P = 0.039; P-选择素:5673±1553P = 0.017)。结论:慢性眼表疾病的多种AqH细胞因子水平升高。

PURPOSE:To evaluate cytokine and protein levels in the aqueous humor (AqH) of eyes with ocular surface diseases.DESIGN:Prospective consecutive case series.METHODS:This study includes 14 patients (aged 62.4 ± 13.7 years) with chronic-phase ocular surface diseases (4 with ocular cicatricial pemphigoid, 5 with chemical burns, 2 with a thermal burn, 2 with Stevens-Johnson syndrome, and 1 with exposure keratitis), 14 matched patients without ocular surface disease (controls with corneal scar), and 30 patients who underwent cataract surgery (healthy controls). AqH samples were collected at the beginning of surgery. AqH levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, monocyte chemotactic protein [MCP]-1, interferon [IFN]-α, IFN-γ, macrophage inflammatory protein [MIP]-1α, MIP-1β, P-selectin, E-selectin, soluble-intercellular adhesion molecule [s-ICAM]-1, tumor necrosis factor [TNF]-α, granulocyte-macrophage colony-stimulating factor [GM-CSF], IFN-γ-induced protein [IP]-10) were measured using multiplex beads immunoassays.RESULTS:The levels of IL-6, IL-10, IL-17A, GM-CSF, E-selectin, P-selectin, and s-ICAM in AqH were significantly elevated in eyes with ocular surface diseases (in pg/mL: 1696 ± 804, 4.0 ± 1.0, 24.3 ± 9.8, 26.0 ± 18.3, 5150 ± 1232, 13122 ± 7219, and 7914±2813, respectively), compared to healthy controls (IL-6: 6.36 ± 0.94, P= .001; IL-10: 1.68 ± 0.04, P=.0006; IL-17A: 3.7 ± 0.2, P= .008; GM-CSF: 2.7 ± 0.3, P= .007; E-selectin: 2093 ± 37, P=.0001; P-selectin: 3658 ± 137, P= .0001; sICAM-1: 1397 ± 119, P= .008). The levels of IL-6, IL-17A, E-selectin, and P-selectin in AqH were significantly higher in eyes with ocular surface diseases compared to those with corneal scar (IL-6: 44.1 ± 15.0, P= .0077; IL-17A: 4.1 ± 0.7, P= .034; E-selectin: 2439 ± 302, P= .039; and P-selectin: 5673 ± 1553, P= .017).CONCLUSIONS:Multiple AqH cytokine levels were elevated in chronic ocular surface diseases.


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