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年龄和性别对健康成人眼表微生物的影响

Wen, X., Miao, L., Deng, Y., et al.

期刊名称:Investigative Ophthalmology & Visual Science

卷期:2017年第58卷第14期

摘要

摘要:目的:越来越多的证据表明,眼表微生物具有强大的免疫调节功能,在健康眼生理维持和眼部疾病发病机制中起着关键作用。尽管已知微生物受到年龄和性别的影响,但是这些因素对健康成年人眼表微生物群的影响至今尚不清楚。方法:从中山眼科中心48名年轻人和42名老年人的下球结膜获得眼表微生物样品。采用宏基因组鸟枪法测序,对健康成人结膜微生物组学特征的性别和年龄差异进行了特征分析。结果:男性和女性组仅在细菌群落的β多样性方面存在差异,而青年组和老年组之间的细菌构成,代谢功能和抗生素抗性基因的丰度存在显着差异。结论:我们的研究结果表明,年龄和性别共同塑造了结膜微生物组,并可能通过眼表面共生微生物的改变而改变眼的免疫稳态。

Purpose:A growing body of evidence suggests that the microbiome of the ocular surface confers potent immunoregulatory functions and has a key role in the physiologic maintenance of healthy eyes and in the pathogenesis of ocular diseases. Although the microbiome is known to be affected by age and sex, the influence of these factors on ocular surface microbiota in healthy adults remains largely unknown.Methods:Ocular surface microbiome samples were obtained from the inferior bulbar conjunctiva of 48 young and 42 old adults at Zhongshan Ophthalmic Center. Using metagenomic shotgun sequencing, we characterized the sex- and age-differences in conjunctival microbiome profiles of healthy adults.Results:Male and female groups differed only in the β diversity of bacterial communities, while there were significant differences in bacterial composition, metabolic functions, and the abundance of antibiotic resistance genes between young and old adult groups.Conclusions:Our findings suggest that age and sex collectively shape the conjunctival microbiome, and may change the immune homeostasis of the ocular surface through alterations of its commensal microbiome.


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