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糖尿病大鼠视网膜系统和谷胱甘肽早期变化

Carpi-Santos R, Ferreira MJ, Pereira NAD, et al.

期刊名称:Experimental Eye Research

卷期:2016年第145卷

摘要

糖尿病视网膜病变(DR)是失明糖尿病患者中的主要原因,主要影响视网膜神经元和血管细胞。有研究表明,神经元细胞在糖尿病后4周开始出现死亡,并可能与氧化应激增加相关。系统[公式:见下文]是一种谷氨酸/胱氨酸交换器,由一种称为XCT的催化亚基和一种调节亚基4F2hc(其活性对谷胱甘肽的合成非常重要,是细胞抗氧化分子的关键)合成。虽然一些研究已经表明,糖尿病大鼠兴奋性氨基酸转运(EAATs)介导的谷氨酸转运出现下调,目前没有在这种情况下的研究这种系统的研究。为了评估这种系统是否受糖尿病早期发病影响,研究人员使用了暴露于高血糖的原发性视网膜细胞培养和注射链脲佐菌素3周后的大鼠视网膜。我们发现,培养物和体内xCT亚基蛋白的表达降低。此外,系统活性和GSH水平也明显下降,而糖尿病动物视网膜的氧化应激增加。因此,本研究提出了一个可能性,即系统表达和活性的变化可能发生于糖尿病发病早期。以这种方式,系统修饰可能与糖尿病性视网膜病的ROS增加有关。

Diabetic retinopathy (DR), the main cause of blindness among diabetic patients, affects both neuronal and vascular cells of the retina. Studies show that neuronal cell death begins after 4 weeks of diabetes and could be related with an increase in oxidative stress. System [Formula: see text] is a glutamate/cystine exchanger, formed by a catalytic subunit called xCT and a regulatory subunit 4F2hc, whose activity is crucial to the synthesis of glutathione, which is a key antioxidant molecule for cells. Although some studies have shown that glutamate transport mediated by excitatory amino acid transporters (EAATs) in diabetic rats is downregulated, there are no studies investigating system [Formula: see text] in this context. To evaluate whether system [Formula: see text] is modified by early onset of diabetes, primary retinal cell culture exposed to high glucose and retinas of rats 3 weeks after streptozotocin injection were used. We observed that xCT subunit protein expression both in cultures and in vivo were diminished. Furthermore, system [Formula: see text] activity and GSH levels were also decreased whereas oxidative stress was increased in retinas of diabetic animals. Therefore, this study raises the possibility that alterations in system [Formula: see text] expression and activity could occur during early onset of diabetes. In that way, system [Formula: see text] modifications could be related to increased ROS in diabetic retinopathy.

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